2023年
Ultrasound in Medicine and Biology (2023-02-01)
Ultrasound Stimulation Potentiates Management of Diabetic Hyperglycemia
生醫所 陳榮治 (通訊作者) / JIF:3.694 / Rank:Q1 (21.8 percent)
Glucose homeostasis is the only way to manage diabetic progression as all medications used do not cure diabetes. This study was aimed at verifying the feasibility of lowering glucose with non-invasive ultrasonic stimulation.The ultrasonic device was homemade and controlled via a mobile application on the smartphone. Diabetes was induced in Sprague-Dawley rats through high-fat diets followed by streptozotocin injection. The treated acupoint CV12 was at the middle of the xiphoid and umbilicus of the diabetic rats. Parameters of ultrasonic stimulation were an operating frequency of 1 MHz, pulse repetition frequency of 15 Hz, duty cycle of 10 percent and sonication time of 30 min for a single treatment. The diabetic rats exhibited a significant decrease of 11.5 percent± 3.6 percent in blood glucose in 5 min of ultrasonic stimulation (p < 0.001). After the single treatment on the first day, third day and fifth day in the first week, the treated diabetic rats had a significantly small area under the curve of the glucose tolerance test (p < 0.05) compared with the untreated diabetic rats in the sixth week. Hematological analyses indicated that the serum concentrations of β-endorphin were significantly increased by 58 percent ± 71.9 percent (p < 0.05) and the insulin level was increased by 56 percent ± 88.2 percent (p = 0.15) without statistical significance after a single treatment. Therefore, non-invasive ultrasound stimulation at an appropriate dose can produce a hypoglycemic effect and improve glucose tolerance for glucose homeostasis and may play a role as adjuvant therapy with diabetic medications in the future.
生醫所 陳榮治 (通訊作者) / JIF:3.694 / Rank:Q1 (21.8 percent)
Glucose homeostasis is the only way to manage diabetic progression as all medications used do not cure diabetes. This study was aimed at verifying the feasibility of lowering glucose with non-invasive ultrasonic stimulation.The ultrasonic device was homemade and controlled via a mobile application on the smartphone. Diabetes was induced in Sprague-Dawley rats through high-fat diets followed by streptozotocin injection. The treated acupoint CV12 was at the middle of the xiphoid and umbilicus of the diabetic rats. Parameters of ultrasonic stimulation were an operating frequency of 1 MHz, pulse repetition frequency of 15 Hz, duty cycle of 10 percent and sonication time of 30 min for a single treatment. The diabetic rats exhibited a significant decrease of 11.5 percent± 3.6 percent in blood glucose in 5 min of ultrasonic stimulation (p < 0.001). After the single treatment on the first day, third day and fifth day in the first week, the treated diabetic rats had a significantly small area under the curve of the glucose tolerance test (p < 0.05) compared with the untreated diabetic rats in the sixth week. Hematological analyses indicated that the serum concentrations of β-endorphin were significantly increased by 58 percent ± 71.9 percent (p < 0.05) and the insulin level was increased by 56 percent ± 88.2 percent (p = 0.15) without statistical significance after a single treatment. Therefore, non-invasive ultrasound stimulation at an appropriate dose can produce a hypoglycemic effect and improve glucose tolerance for glucose homeostasis and may play a role as adjuvant therapy with diabetic medications in the future.
Talanta (2023-03-01)
Application of Aminobenzoic Acid Electrodeposited Screen-printed Carbon Electrode in the beta-amyloid Electrochemical Impedance Spectroscopy Immunoassay
生醫所 陳榮治 (通訊作者) / JIF:6.556 / Rank:Q1 (12.7 percent)
Alzheimer's disease (AD) is one of the important neurodegenerative diseases, in the modern aging society, it has become an issue people need to work on. Of the pathogenic factor which leads to AD, beta-amyloid (Aβ) is the most important one. It can form the senile plaque which aggregates in the neuron and interrupts the signal transmission. This research is based on the electrochemical system and screen-printed carbon electrode (SPCE) incorporated with pretreatment, electrodeposition, electrochemical impedance spectroscopy (EIS), antibody, and blocking agent. This immunosensor is applied to detect the different concentrations of Aβ. The standard curve between electrical impedance and concentration of Aβ is calculated. The specificity of the immunosensor is tested. This survey optimizes the electrodeposition condition for 4-aminobenzoic acid (4-ABA) and the parameter for antibody and blocking agents. This study fabricates a more dense, uniform, and stable film of 4-ABA. This sensor presents a range of detection from 1 fg/ml to 100 pg/ml and a limit of detection to 3.84 fg/ml. This sensor can identify the isoform of Aβ. This research shortens the fabricating time to 3.5 h. This study fabricates a label-free and low-cost immunosensor for Aβ with a short fabricating time, high stability, wide range of detection, low limit of detection, and good specificity. The impedance of the carbon printed electrodes is very high and is always measured by its current but this study provides a fabrication technique for high-efficiency carbon printed electrodes for electrochemical impedance spectroscopy sensing.
生醫所 陳榮治 (通訊作者) / JIF:6.556 / Rank:Q1 (12.7 percent)
Alzheimer's disease (AD) is one of the important neurodegenerative diseases, in the modern aging society, it has become an issue people need to work on. Of the pathogenic factor which leads to AD, beta-amyloid (Aβ) is the most important one. It can form the senile plaque which aggregates in the neuron and interrupts the signal transmission. This research is based on the electrochemical system and screen-printed carbon electrode (SPCE) incorporated with pretreatment, electrodeposition, electrochemical impedance spectroscopy (EIS), antibody, and blocking agent. This immunosensor is applied to detect the different concentrations of Aβ. The standard curve between electrical impedance and concentration of Aβ is calculated. The specificity of the immunosensor is tested. This survey optimizes the electrodeposition condition for 4-aminobenzoic acid (4-ABA) and the parameter for antibody and blocking agents. This study fabricates a more dense, uniform, and stable film of 4-ABA. This sensor presents a range of detection from 1 fg/ml to 100 pg/ml and a limit of detection to 3.84 fg/ml. This sensor can identify the isoform of Aβ. This research shortens the fabricating time to 3.5 h. This study fabricates a label-free and low-cost immunosensor for Aβ with a short fabricating time, high stability, wide range of detection, low limit of detection, and good specificity. The impedance of the carbon printed electrodes is very high and is always measured by its current but this study provides a fabrication technique for high-efficiency carbon printed electrodes for electrochemical impedance spectroscopy sensing.
Biosensors and Bioelectronics (2023-05-23)
Rapid detection of live bacteria in water using nylon filter membrane-integrated centrifugal microfluidics
生醫所 李博仁 (通訊作者) / JIF:12.545 / Rank:Top 3.45 percent (3/87)(CHEMISTRY, ANALYTICAL)
Water is one of the most indispensable elements for human beings. People can live without food for a couple of weeks but cannot live without water for a couple of days. Unfortunately, drinking water is not always safe around the world; in many areas, the water for drinking could be contaminated with various microbes. Therefore, in this work, we report a novel, simple, and highly efficient strategy to detect live bacteria in water via a nylon membrane-integrated centrifugal microfluidic device. We highly anticipate that this platform can improve water quality monitoring in resource-poor countries soon.
生醫所 李博仁 (通訊作者) / JIF:12.545 / Rank:Top 3.45 percent (3/87)(CHEMISTRY, ANALYTICAL)
Water is one of the most indispensable elements for human beings. People can live without food for a couple of weeks but cannot live without water for a couple of days. Unfortunately, drinking water is not always safe around the world; in many areas, the water for drinking could be contaminated with various microbes. Therefore, in this work, we report a novel, simple, and highly efficient strategy to detect live bacteria in water via a nylon membrane-integrated centrifugal microfluidic device. We highly anticipate that this platform can improve water quality monitoring in resource-poor countries soon.
Journal of Biophotonics (2023-06-06)
Intraoperative application of optical coherence tomography for lung tumor
生醫所 孫家偉 (通訊作者) / JIF:2.8 / Rank:"46/100 46 percent OPTICS"
On-site instant determination of benign or malignant tumors for deciding the types of resection is crucial during pulmonary surgery. We designed a portable spectral-domain optical coherence tomography (SD-OCT) system to do real-time scanning intraoperatively for the distinction of fresh tumor specimens in the lung. A total of 12 ex vivo lung specimens from six patients were enrolled. Three patients were diagnosed with invasive adenocarcinoma (IA), while the others were benign. After OCT-imaged reconstruction, we compared the qualitative morphology of OCT and histology among malignant, benign, and normal tissues. In addition, through analysis of the quantitative data, a discrete difference in optical attenuation coefficients around the junctional surface was shown by our data processing. This study demonstrated a feasible OCT-assisted resection guide by a rapid on-site tumor diagnosis. The results indicate that future deep learning of OCT-captured image systems able to improve diagnostic and therapeutic efficiency is warranted.
生醫所 孫家偉 (通訊作者) / JIF:2.8 / Rank:"46/100 46 percent OPTICS"
On-site instant determination of benign or malignant tumors for deciding the types of resection is crucial during pulmonary surgery. We designed a portable spectral-domain optical coherence tomography (SD-OCT) system to do real-time scanning intraoperatively for the distinction of fresh tumor specimens in the lung. A total of 12 ex vivo lung specimens from six patients were enrolled. Three patients were diagnosed with invasive adenocarcinoma (IA), while the others were benign. After OCT-imaged reconstruction, we compared the qualitative morphology of OCT and histology among malignant, benign, and normal tissues. In addition, through analysis of the quantitative data, a discrete difference in optical attenuation coefficients around the junctional surface was shown by our data processing. This study demonstrated a feasible OCT-assisted resection guide by a rapid on-site tumor diagnosis. The results indicate that future deep learning of OCT-captured image systems able to improve diagnostic and therapeutic efficiency is warranted.
Biosensors and Bioelectronics (2023-09-15)
Rapid detection of live bacteria in water using nylon filter membrane-integrated centrifugal microfluidics
生醫所 李博仁 (通訊作者) / JIF:12.6 / Rank:0.979
Our platform provides a convenient and sensitive strategy for rapid monitoring. We highly anticipate that this platform can improve water quality monitoring in resource-poor countries in the near future.
生醫所 李博仁 (通訊作者) / JIF:12.6 / Rank:0.979
Our platform provides a convenient and sensitive strategy for rapid monitoring. We highly anticipate that this platform can improve water quality monitoring in resource-poor countries in the near future.
Iscience (2023-08-18)
Design of a gradient-rheotaxis microfluidic chip for sorting of high-quality Sperm with progressive motility
生醫所 李博仁 (通訊作者) / JIF:5.8 / Rank:0.801
Progressive Sperm Sorting in a microfluidic chip •Low DNA damage and high integrity morphology •Shorter sorting time compared to traditional sperm sorting methods
生醫所 李博仁 (通訊作者) / JIF:5.8 / Rank:0.801
Progressive Sperm Sorting in a microfluidic chip •Low DNA damage and high integrity morphology •Shorter sorting time compared to traditional sperm sorting methods
